Go ahead and test it like you would any load.
I can't speak for the .38 special. But many a cotton mouth went to it's maker from shot out of my .44 mag. Ruger Redhawk. I don't remember the charge but I used #7 1/2 shot and it was very effective. It would reach quite a ways out on my ponds at my farm. These shot capsules to produce a dognut shaped pattern however. For snakes I simply aimed for the center of the body and often hit it in the head and rear regions.
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